COMPARISON, VALIDATION, AND OPTIMIZATION OF INTERNAL GENOMIC DNA EXTRACTION PROTOCOL FOR CAMPYLOBACTER SPECIES

Campylobacter remains the leading cause responsible of human gastroenteritis worldwide. The current study aimed to compare internal DNA extraction protocol with two commercially available kits, using C. jejuni (ATCC® 29428TM) and coli (ATCC®43478TM) validate optimize through artificial contamination confirmation spp. from broiler chickens, turkeys, beef meats samples. processes were carried out following protocols steps manufacturer's instructions PureLinkTM Genomic Mini Kit Wizard® Purification Kit, respectively. agarose gel electrophoresis system was used control quality. After that, forty isolates confirmed, finally, an aforementioned reference strains at three different concentrations performed sterilized minced meats. This is first work comparing genomic for results indicated that provided similar efficiency kits. All confirmed successfully amplified, in which28 12 jejuni, as revealed by biochemical tests. A positive amplification also observed contaminated food matrices, after enrichment, all examined doses. Except some reactions negative 1 CFU/mL coli. explained detection limits both qPCR. Based on our findings, crucial determining quality this amended. Hence, highlighted importance validating simpler, cheaper faster protocol, each laboratory, part future risk assessment, monitoring programs frequency required molecular studies.